other:Article Title: Metformin Induces Apoptosis and Ferroptosis of Ovarian Cancer Cells Under Energy Stress Conditions
Article Snippet: The antibodies are as follows: Caspase 3 antibody (Cat. 19677-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), Caspase 9 antibody (Cat.10380-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), β-actin antibody (Cat. 81115-1-RR, 1:2000, Proteintech, Wuhan, Hubei Province, China), GPX4 antibody (Cat. 67763-1-Ig, 1:1000, Proteintech, Wuhan, Hubei Province, China), Bax antibody (Cat. 50599-2-Ig, 1:1000, Proteintech, Wuhan, Hubei Province, China), FSP1 antibody (Cat. 20886-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), NRF2 antibody (Cat. 16396-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), Bcl2 antibody (Cat. 68103-1-Ig, 1:1000, Proteintech, Wuhan, Hubei Province, China), IDH1 antibody (Cat. 12332-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), NDUFB4 antibody (Cat. 27931-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), PDH E1 Alpha antibody (Cat. 18068-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), ACSL4 antibody (Cat. 22401-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), NCOA4 (E8H8Z) antibody (Cat. 66849, 1:1000, Cell Signaling Technology, Danvers, MAUSA), Apaf1 antibody (Cat. 29022-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China).
Article Title: Metformin Induces Apoptosis and Ferroptosis of Ovarian Cancer Cells Under Energy Stress Conditions
Article Snippet: The antibodies are as follows: Caspase 3 antibody (Cat. 19677-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), Caspase 9 antibody (Cat.10380-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), β-actin antibody (Cat. 81115-1-RR, 1:2000, Proteintech, Wuhan, Hubei Province, China), GPX4 antibody (Cat. 67763-1-Ig, 1:1000, Proteintech, Wuhan, Hubei Province, China), Bax antibody (Cat. 50599-2-Ig, 1:1000, Proteintech, Wuhan, Hubei Province, China), FSP1 antibody (Cat. 20886-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), NRF2 antibody (Cat. 16396-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), Bcl2 antibody (Cat. 68103-1-Ig, 1:1000, Proteintech, Wuhan, Hubei Province, China), IDH1 antibody (Cat. 12332-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), NDUFB4 antibody (Cat. 27931-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), PDH E1 Alpha antibody (Cat. 18068-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), ACSL4 antibody (Cat. 22401-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China), NCOA4 (E8H8Z) antibody (Cat. 66849, 1:1000, Cell Signaling Technology, Danvers, MAUSA), Apaf1 antibody (Cat. 29022-1-AP, 1:1000, Proteintech, Wuhan, Hubei Province, China).
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![Mitochondrial metabolism and neutrophil response in varied calcium availability. (A) Immunoblot of <t>phospho-PDH[ser293],</t> phospho-PDH[ser300], total PDH E1 subunit, and β-actin in differentiated ER-Hoxb8 neutrophils stimulated with ionomycin for indicated duration. Relative signals (normalized by β-actin from the same blot) from 4 different independent experiments were made relative to the unstimulated condition and compiled. Representative images of immunoblots are shown to the right. Significance was determined with RM two-way ANOVA with Geisser-Greenhouse correction and Šídák’s multiple comparisons test. (B) Labeling pattern of glycolysis and TCA intermediates from U 13 C-glucose in primary human neutrophils treated under specified conditions for 1 hour. Data shown are representative of experiments from 3 different human donors. (C) Extracellular DNA release over time of primary human neutrophils cultured under specified conditions. Representative microscopy images at the 4.5-hour time point are shown to the right. Data shown are representative of experiments from 3 different human donors. (D) MPO activity assay from supernatant of primary human neutrophils treated for 4 hours under specified conditions. Data shown are representative of experiments from 3 different human donors. Significance was determined by ordinary one-way ANOVA and Šídák’s multiple comparisons test. (E) Number of neutrophils that migrated through a transwell to C5a after 1.5 hours under specified treatment conditions. Data shown are representative of experiments from 3 different human donors. Significance was determined by unpaired t test. Statistical significance is indicated by the following annotations: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6771/pmc12066771/pmc12066771__fimmu-16-1572927-g003.jpg)